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Part:BBa_J100308

Designed by: Monica Prudencio   Group: Campbell M Lab   (2016-10-10)


Variant of repClone Red(J100205) w/ wt TetR promoter (R0040)

repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. This will allow researchers and biology students to explore sequence requirements for the interactions between the TetR repressible promoter, TetR protein, and aTc.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 948
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2213
    Illegal AgeI site found at 2325
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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